Generation of three induced pluripotent stem cell lines (RAUi001-A, RAUi001-B and RAUi001-C) from peripheral blood mononuclear cells of a healthy Armenian individual
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BiBTeXThe study of pathological processes in cells carrying mutations should be carried out in comparison with a healthy control group. Familial Mediterranean fever (FMF), which is caused by a mutation in the MEFV gene, is predominantly found in people of Armenian nationality with the prevalence of 14–100 per 10000. We have obtained induced pluripotent stem cells (iPSCs) from Armenian healthy patient, which will be included as a control group in the study of this disease. iPSCs rapidly proliferate in colonies of cells with a typical pluripotent-like morphology, have a normal karyotype (46,XX). iPSCs express pluripotency markers (OCT4, SOX2, TRA-1–60, NANOG) and are able to give derivatives of three germ layers.
SEEK ID: https://armlifebank.am/publications/18
DOI: 10.1016/j.scr.2023.103147
Projects: Study of the molecular mechanisms of familial Mediterranean fever using ...
Publication type: Journal
Journal: Stem Cell Research
Publisher: Elsevier BV
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Created: 6th Dec 2024 at 09:23
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Institutions: Institute of Molecular Biology NAS RA, Armenian Bioinformatics Institute
https://orcid.org/0000-0002-6851-1056
Disorders within the cells, the fundamental building blocks of life, lie at the core of all human diseases. This program provides information on various projects aimed at improving human health, possibly as diagnostic, preventive or curative therapies. These are collaborations with several research institutes supported by various grant programs.
Web page: Not specified
In this project genetic engineering and functional genomics approaches will be used to describe molecular mechanisms of FMF. Using DNA/RNA samples obtained from FMF patients and healthy subjects MEFV nanopore gene sequencing will be performed to identify genetic variants and possibly find novel putative functional variants. The impact of genetic variants on transcriptome landscape will be studies by RNA-sequencing using nanopore sequencing device. Based on MEFV sequencing data and patients clinical ...
Programme: Cellular basis of diseases
Public web page: Not specified
Start date: 1st Sep 2021
End date: 31st Aug 2024
